Antimicrobial function of SHβAP, a novel hemoglobin β chain-related antimicrobial peptide, isolated from the liver of skipjack tuna, Katsuwonus pelamis.

Seo JK, Lee MJ, Jung HG, Go HJ, Kim YJ, Park NG., Fish Shellfish Immunol., 2014, Feb 2.  doi: 10.1016/j.fsi.2014.01.021

A 2.3 kDa of antimicrobial peptide was purified from an acidified liver extract of skipjack tuna, Katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and C₁₈ reversed-phase HPLC. A comparison of the amino acid sequence of the purified peptide with those of other known polypeptides revealed high homology with the C-terminus of hemoglobin β-chain; thus, this peptide was designated as the Skipjack Hemoglobin β chain-related Antimicrobial Peptide(SHβAP). SHβAP showed potent antimicrobial activity against Gram-positive bacteria, such as Bacillus subtilis, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentrations [MECs], 6.5-57.0 Î¼g/mL), Gram-negative bacteria, such as Escherichia coli D31, Pseudomonas aeruginosa, Salmonella enterica, Shigella sonnei, and two Vibrio parahaemolyticus species (MECs, 2.0-19.0 Î¼g/mL), and against Candida albicans (MEC; 12.0 Î¼g/mL) without significant hemolytic activity. Antimicrobialactivity of this peptide was heatstable and pH resistant but is sensitive to proteases and salt. SHβAP did not show membrane permeabilization and killing ability. The secondary structural prediction and the homology modeling expected that this peptideformed an amphipathic α-helical structure. This is the first report the purification of a novel antimicrobial peptide related to the C-terminus of hemoglobin β-chain from marine fish.

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在北京时间8月30日，根据Stat Muse的数据统计显示，金州勇士队的篮球明星斯蒂芬-库里在多项统计数据上均位列球队历史首位。

总得分数

季后赛总得分数

总助攻数

总抢断数

总投篮命中数

季后赛总投篮命中数

总三分命中数

季后赛总三分命中数

总胜场数

生涯至今，库里率领球队四夺总冠军，荣膺两次MVP&一次FMVP&两次得分王，10次入选全明星(1次MVP)，10次入选NBA最佳阵容(4次一阵)，加冕NBA历史三分王并入选NBA75大球星。

Mar
01

This article is available courtesy of Caliper.

If classic movies like Glengarry Glen Ross (1992), Tin Men (1987), Used Cars (1980)—or more recent hits like Wolf of Wall Street (2013)—have taught us anything, it’s that sales is not for those of a weak disposition.

And whether the character portrayal is ruthless and intimidating like Alec Baldwin’s Blake, broadly comedic like Danny DeVito’s Ernest Tilley, or charmingly sneaky like Kurt Russell’s Rudy Russo, movies have also suggested that salespeople need to be either pushy or conniving to achieve success.

While such representations do elevate dramatic tension or provide an impetus for farcical scenarios to play out on screen, they’re pretty far from reality, especially in today’s world of sales. Now more than ever, customers are informed and have a wide array of choices. Online reviews, comparison websites, specs, even satellite and street views of real estate are at the fingertips of potential buyers. So if salespeople have lost their leverage with information, what can they do to win the account?

Some attributes typically associated with sales remain important and probably will be for some time. Building relationships, for example, is something even the most advanced predictive algorithm cannot do. Persuasiveness, coupled with social engagement (which we might describe as “personal impact”), is also a major component of selling. As implied in the opening paragraph above, resilience is perhaps most critical of all to sticking it out in competitive sales. The best salesperson loses at least 75% of the time.

Alas, personal impact and resilience are not teachable qualities. There are compensatory techniques that can be conveyed through training and coaching, but both psychometric research and practical experience show that such techniques are rarely sustainable in sales. If you work against your intrinsic motivations, like cold-calling when you are socially reserved and lack self-assurance for example, you are likely to burn out sooner rather than later.

So let us assume we’re addressing salespeople who already display personal impact, are adept at relationship building, and can rebound from rejection. How can they adapt in a sales environment where information technology has empowered customers as never before? By demonstrating the fourth common success factor revealed by psychometric research: curiosity.

In measuring the personality drivers of successful sales people across multiple industries, we have found that “information seeking” is a quality shared among many top performers. We’re not talking about following a sales script or asking basic qualifying questions. Information seekers are naturally curious about people and things. Information seekers are not dismissive of new ideas and input, either, and they are open to others’ perspectives and experiences. Your grumpy uncle who reflexively thinks anything that does not interest him is a stupid waste of time? Not an information seeker. Maybe he did great in insurance sales thirty or forty years ago, but he’d find a very different environment today.

How does information-seeking lead to sales success? Now that’s a question for curious people! The folks who bailed on this piece after reading the previous paragraph—because they reflexively dismissed the concept—were not information seekers either. Let’s explore the answer without them.

Information seeking supports sales success because it redirects the conversation. The customer did the research and already has the specs on hand and prices in mind. They think they are in control. When you start asking questions—meaningful, probing questions they weren’t expecting—the information you elicit gets them thinking and rethinking, perhaps about potentialities they overlooked. You’re showing them that you have a whole level of understanding they hadn’t imagined, giving you instant credibility. Now they’re more receptive to the message you intended to convey all along.

Information seeking doesn’t always manifest in the form of overt questions. It could be quiet observation, or it could be collecting seemingly irrelevant data that you process in the back of your mind. It could be simple environmental scanning. Salespeople who are open to such inputs are the ones who, once the clues coalesce in their minds, find the right button to push or notice hidden opportunities that turn a minor sale into a major one.

Contrast that with the “old-school” sales approach, in which the sales representative already has his mind made up about what clients want and need and shuts down any chance of engagement. He might be friendly, persuasive, and resilient to setbacks, but his close-minded, inflexible approach leaves money on the table.

Curiosity in sales isn’t a new thing, of course, but simply an attribute that hasn’t always been considered in the applicant-selection process. Recent performance data says it should be. So consider stirring in a little Sherlock Homes with your Glengarry Glen Ross. Or should we call it, perhaps, The Case of the Missing Leads?

To learn more about Caliper and special discounts to NFDA members, visit www.calipercorp.com/cisca.

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The daily maintenance of the screw barrel is a problem that many people in the industry are concerned about, so what are the specifics?

1.The predetermined temperature of the screw element

Do not start the machine when the screw element has not reached the .

2. Prevent metal fragments and debris from falling into the hopper of the screw element of the extruder. 

If processing recycled materials, a magnetic hopper must be added to prevent iron filings from entering the barrel.

3. Make sure that the plastic in the screw element barrel is completely melted 

Make sure that the plastic in the extruder screw elements barrel is completely melted when using anti-salvation, so as not to damage the transmission system parts when the screw element retreats.

4. The remaining material of the screw element barrel should be cleaned

When using POM, PVC, PA+GF and other materials, minimize the degradation of the raw materials, and rinse them with ABS and other nozzle materials in time after shutdown.

5. The molten plastic

When the temperature of the molten plastic is normal, but black spots or discoloration of the molten plastic are constantly found, check whether the screw non-return ring (over apron, meson) is damaged.

6. Plasticization of the screw element

During processing, try to make the material in the barrel of the screw element plasticize evenly, do not let metal foreign matter mix into the material, and reduce the rotating torque of the barrel of the screw element.

7. Avoid the phenomenon of screw idling and slipping

If the machine stops for more than half an hour each time, it is best to close the blanking port and clean the material in the barrel, and set up heat preservation. Avoid the phenomenon of screw idling and slipping.

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Josh Mart takes the chance to preview the second leg of this year’s UEFA Champions League round of 16 between FC Barcelona and Paris Saint Germain at the Camp Nou in Barcelona.

The first leg was nothing short of remarkable. PSG defied all the odds and managed to thrash the Catalonian giants by four goals to nil, a scoreline which was thoroughly deserved and reverberated around the football world. PSG were on to Barcelona straight from the kick off and were utterly relentless. Goals from Edinson Cavani, Julian Draxler and an Angel Di Maria double were the least the Parisians deserved even if Barca didn’t perform at anywhere near their best.

The second leg tonight promises to be interesting to say the least as Barcelona look for revenge at Camp Nou. Most online tipsters, unsurprisingly, are backing Barcelona for the win but with outside odds of 21/5 for PSG to cause an upset, some might fancy the away side finally getting one over on the team which has been their proverbial “thorn in the side” for years.

Since the heavy defeat in Paris, Barcelona have been near perfect domestically scoring a mightily impressive 15th goal in their last 4 games. Saturday’s 5-0 thrashing of Celta Vigo was considered by some to be Barcelona’s most complete performance of the season.

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This coincides with Luis Enrique’s decision to switch to a 3-4-3 formation which could be deployed against PSG tonight. Coach Luis Enrique has recently announced that he will be leaving Barcelona at the end of the season, but he and his squad will be desperate to make history and pull of the near impossible at the Camp Nou this evening.

PSG have also been in great form in France, despite a minor European hangover after the Barcelona rout where they drew 0-0 at home to Toulouse. A fabulous 5-1 victory in ‘Les Clasique’ away to Marseille has been followed up by a Coupe de France win over Niort and a routine 1-0 victory over Nancy at the Parc des Princes on Saturday.

Despite their good form, Unai Emery’s men still behind leaders Monaco in the Ligue 1 standings but their spectacular performance against Barcelona has gone some way to silencing Emery’s critics this season.

Team News and Tactics:

Barcelona are without the injured duo of Aleix Vidal and Jeremie Mathieu for this one, but Luis Enrique’s main decision to make is whether to play the 3-4-3 formation which worked so well against Celta at the weekend, or revert to the clubs typical 4-3-3 formation.

I think that Barcelona will line up with the 3-4-3 with Ter Stegen behind a back 3 of Mascherano, Pique and Umtiti. The midfield will be quite narrow to try and gain more control of the ball- something which they lacked terribly at the Parc des Princes. Sergio Busquets and Andres Iniesta look set to start and will most probably be joined by Ivan Rakitic and one of Jordi Alba, Sergi Roberto or Andre Gomes depending on how much Enrique wants from the midfield. The front 3 of Messi, Suarez and Neymar picks itself, despite a very under par performance from them all 3 weeks ago.

Unai Emery really does have some big selection problems here, as he is missing Thiago Motta and probably Di Maria through injury and there has been speculation about Marco Verratti and Adrien Rabiot missing out too.

With this in mind, I expect Unai Emery to stick with his 4-3-3 formation with Trapp in goals behind a defence of Meunier, Marquinhos, Thiago Silva and Kurzawa. Kimpembe could retain his place but the experience of Thiago Silva may be needed here. If the injury rumours are to be believed it would be very tricky to assume PSG’s midfield trio, but I think at least one of Verratti/Rabiot will play alongside Matuidi in midfield. The other option (if needed) could be filled by Krychowiak or the talented youngster Nkunku. Di Maria’s void is likely to be filled by Lucas Moura, who will play in the front three alongside Julian Draxler and Edinson Cavani.

Danger Men

Lionel Messi (Barcelona) – He had a very unusually poor evening in Paris in the first leg, although he wasn’t alone in that regard. He has been in scintillating form since then and is said to be full of determination and desire to make history and overturn this monumental deficit in this game. If anyone can…

Julian Draxler (PSG) – Julian Draxler’s career promised so much when he broke through and became an integral part of the first team at Schalke but he was criticised heavily (partly justifiably) for what was seen as a sideways move to Wolfsburg. Since moving to Paris though, he has been superb. The talent which has never been in question is now accompanied by a hunger and desire to win which was so evident in the first leg.

Prediction

This is a very strange game to predict because it is not often that there is such a large margin in the aggregate scores at this level of competition. It’s hard not to see Barcelona winning, purely because PSG have no need to try and win the match and because they will know that their pride is at stake here. I think Barcelona will win the game, but PSG will go through thanks to their marvellous first leg performance.

Prediction: Barcelona 3-1 PSG

From 20th to 22nd March, Engineering & Machinery Asia was held in Karachi, Pakistan as scheduled. Nearly 1,000 exhibitors gathered from all over the world at the Karachi Expo Center. Since the exhibition was held, its popularity has been growing year by year, and more and more Chinese companies have taken the opportunity to join the exhibition. The block machine of  Quangong Machinery Co., Ltd. has been ranked at the forefront of market share in South Asia for many years. This time, it also dispatched the Sales elite to attend the exhibition and achieved good results.

 

In recent years, Pakistan’s political situation has stabilized and the economy has entered a period of good development. The government is vigorously developing infrastructure projects, and its ninth five-year plan invests 100 billion rupees (about 1.7 billion US dollars), and strives to transform existing roads and build highways to promote further economic development.

Under this huge demand, Pakistan's demand for block machines is increasing day by day, and the huge building materials market potential is gradually emerging. In recent years, under the "The Belt and Road" initiative, China's trade with Pakistan has become increasingly close. As the second largest economy in South Asia after India and the sixth most populous country in the world, Pakistan’s potential business opportunities cannot be underestimated. The profound traditional friendship between China and Pakistan is a powerful guarantee for friendly economic and trade exchanges. And with the rapid rise of China's construction machinery industry in recent years, "Made in China" has been seen everywhere in Pakistan, and the penetration rate is evident. Quangong Machinery Co., Ltd. is one of the Chinese companies that entered Pakistan earlier. At present, there are many equipment in Pakistan still in operation. With excellent quality and efficiency, the blocks produced by QGM are laid in every city in Pakistan.

 

At this exhibition, the customers who came to visit the booth were in a constant stream. They had an in-depth exchange on the manufacture of concrete products and the marketing representatives of QGM. At the same time, many old customers also came to consult the upgrading of production lines and the introduction of new equipment to cope with the wave of construction in Pakistan in the future.

The deep market potential of the South Asian market is an important part of QGM's development of the global market, and its proportion is very important. In the future, QGM will continue to maintain its product quality and service attitude and strictly demand itself, so that the brand of QGM will resound through the South Asian subcontinent and further enhance the traditional friendship between the two countries. ‍

在北京时间8月21日的最新消息中，波士顿凯尔特人队的球员杰伦-布朗最近接受了NBC记者Monte Poole的采访。

他说：“说实话，上赛季已经结束了。我们庆祝了，我们享受了一段美好的时光，这是一个很棒的夏天——我这个夏天一直都过得很好。但它已经结束了，现在我们要重新回到工作中。”

“现在，我们成了别人的目标，每个人都想战胜我们。那就放马过来吧。所以我们要继续努力工作，我期待着新赛季。”

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Leicester City striker Jamie Vardy allegedly insulted Sevilla’s French attacking midfielder Samir Nasri’s family last night, leading to the Manchester City loanee to react in a movement that was interpreted as a head-butt, according to France Football.

Vardy is said to have insulted the player and his family verbally on numerous occasions leading up to the red card for Nasri. The incident proved fatal for Sevilla who failed to overturn the deficit necessary last night in the second half to progress in the Champions’ League or at least take the match to extra time.

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